Comparison of the point-of-care blood gas analyzer versus the laboratory auto-analyzer for the measurement of electrolytes
© Springer-Verlag London Ltd 2009
Received: 8 October 2008
Accepted: 27 January 2009
Published: 24 February 2009
Electrolyte values are measured both by arterial blood gas (ABG) analyzers and central laboratory auto-analyzers (AA), but a significant time gap exists between the availability of both these results, with the ABG giving faster results than the AA. The authors hypothesized that there is no difference between the results obtained after measurement of electrolytes by the blood gas and auto-analyzers.
After approval by the ethics committee, an observational cohort study was conducted in which 200 paired venous and arterial samples from patients admitted to the Medical Intensive Care Unit (ICU) of Apollo Hospital, Hyderabad, India, were analyzed for electrolytes on the ABG machine and the AA. Analyses were done on the ABL555 blood gas analyzer and the Dade Dimension RxL Max, both located in the central laboratory. Statistical analyses were performed using paired Student’s t test.
A total of 200 paired samples were analyzed. The mean ABG sodium value was 131.28 (SD 7.33), and the mean AA sodium value was 136.45 (SD 6.50) (p < 0.001). The mean ABG potassium value was 3.74 (SD 1.92), and the mean AA potassium value was 3.896 (SD 1.848) (p = 0.2679).
Based on the above analysis, the authors found no significant difference between the potassium values measured by the blood gas machine and the auto-analyzer. However, the difference between the measured sodium was found to be significant. We therefore conclude that critical decisions can be made by trusting the potassium values obtained from the arterial blood gas analysis.
Point-of-care testing enables clinicians to initiate appropriate treatment for emergent conditions, thereby benefitting the patient both clinically and economically .
Electrolyte values are conventionally measured for all critical patients who present to the emergency department, for patients receiving fluid therapy, and for patients admitted to intensive care units (ICU).
Differences between the blood gas analyzer and auto-analyzer
Blood gas analyzer
Analyzes whole blood
Arterial sample used conventionally
Venous sample used conventionally
Uses direct ion selective electrode
Uses indirect ion-selective electrode technology
Uses heparin-diluted sample
Serum sample diluted with fixed volume diluent
Processing time is short
Processing time is long
No effect of protein levels in blood
Affected by protein levels in blood
The United States Clinical Laboratory Improvement Amendment (US CLIA) 2006  accepts a difference of 0.5 mmol/l in measured potassium, and 4 mmol/l in measured sodium, from the gold standard measure of standard calibration solutions.
Previous studies  that measured the accuracy of electrolyte values obtained by ABG machines concluded that the results from two different measurement technologies differed significantly for plasma sodium and chloride concentrations. These differences significantly affected the calculated anion gap and strong ion difference values.
Dilution with heparin raises the volume of the sample, thereby lowering the value of the measured electrolytes on the ABG.
High volume of heparin itself binds the electrolytes, thereby lowering the value of measured electrolytes on the ABG.
Our study had a null hypothesis that there is no difference between the electrolyte measurements by the AA and ABG machines.
Materials and methods
The study was approved by the ethics committee of Apollo Hospitals, Hyderabad, India. The study was designed to be an observational study of a consecutive cohort of patients admitted to the ICU.
Paired blood samples from 200 consecutive adult patients admitted to the Medical ICU of Apollo Hospitals were included in the study. The study period started in August 2005 and ended in May 2006. Blood samples were collected simultaneously for ABG and AA for electrolyte analysis. All samples were analyzed on the ABL555 blood gas analyzer and the Dade Dimension RxL Max, both located in the central laboratory. Both of these are considered as benchmark top models in their respective technologies.
Quality control was ensured by having the blood samples collected by trained staff of a single ICU unit in the hospital and analyzed in the two analyzers located in the central laboratory under similar environmental conditions, unlike in the previous studies where the analyzers were in different environments . Both analyzers were calibrated  according to the Joint Commission on Accreditation of Hospitals (JCAHO) requirements for hospital certification prior to each measurement cycle. Time of collection of samples was documented on both sample groups for reference. Operators drawing and handling blood samples did so with only latex gloves without using any hand disinfectants just prior to handling blood .
The 200 paired samples included a venous blood sample for auto-analyzer electrolyte measurement and an arterial blood sample for blood gas analysis.
Data were collected, and the values of sodium (Na+) and potassium (K+) from these measurements were compared using the paired t-test.
Statistical analysis of sodium samples
Blood gas analyzer
Stratified analysis of difference between Na+ measured by AA and ABG
Mean difference mmol/l
SD difference mmol/l
Max. difference mmo/l
Min. difference mmol/l
Analysis of the sodium measurements in the group of patients with normal sodium revealed a significant difference between the two measures (p = 0.005; null hypothesis rejected). However, the mean difference between the two measuring methods was 3.4 mmol/l, which was within the acceptable limit defined in the US CLIA 2006.
There was no significant difference in sodium measurements by AA and ABG for patients with hypernatremia (mean difference 3.8 mmol/l; p = 0.3847, null hypothesis accepted). However, because of the small sample size (n = 5) in this subgroup, we were underpowered to detect a difference.
In the group of patients with borderline hyponatremia (serum sodium 120–135 mmol/l) and hyponatremia (serum sodium <120 mmol/l), the mean difference between the two measurements was 7.4 mmol/l and 12.8 mmol/l (p < 0.0001 for both subgroups), effectively making the ABG measurements unreliable.
Statistical analysis of potassium
Blood gas analyzer
Stratified analysis of the difference between K+ measured by AA and ABG
No. of values
Mean difference mmol/l
SD difference mmol/l
Max. difference mmo/l
Min. difference mmol/l
The mean differences in patients with hyperkalemia, normokalemia, and hypokalemia were 0.44 mmol/l (SD 0.05), 0.46 mmol/l (SD 0.03), and 0.42 mmol/l (SD 0.02), respectively, all of which are less than the US CLIA 2006 guideline of 0.5 mmol/l.
Point-of-care analyzers are force multipliers for clinicians working in the areas of critical care and emergency medicine. They have been proven to benefit clinical decision-making by reducing the turnaround time for routine biochemical investigations. Time critical decisions are enabled by point-of-care analyzers when biochemical parameters are beyond their normal limits. Several kinds of point-of-care testing equipment are in vogue in clinical practice today, and they have been widely evaluated in varied environments.
Point-of-care testing has gained special favor in emergency transport systems, critical care departments, and cardiothoracic surgical departments, as is shown by the large volume of systems developed for all of these areas [12–14].
However, high operational costs are a deterrent to the application of specialized point-of-care equipment in developing countries . Direct costs have been decreasing as the supply of such analyzers increases. The aim of the study was to evaluate existing point-of-care arterial blood gas analyzers for electrolyte measures. The instrumental difference between the AA and the ABG analyzers is the turnaround time to get the results. Also the fact that blood gas analyzers are unaffected by serum protein levels in their electrolyte measures is an added advantage to using them as a point-of-care electrolyte measure in critically ill patients [16, 17].
Despite the differences that have been cited in the working of the ABG machine and the AA, this study shows that there are no significant differences between the K+ measured by the AA and the ABG machines. The authors therefore suggest that decisions based on serum K+ can be made by trusting the value obtained on the ABG machine.
The study agrees with previous studies  that Na+ values obtained on the ABG machine are not completely reliable when making clinical decisions. Though statistically significant, the difference in the measured Na+ values in hypernatremic and normo-natremic patients was within the recommended US CLIA guidelines. Whether statistical difference also translates to clinical difference remains to be seen.
Some of the limitations of our study are that the results are limited to the use of one AA and one ABG machine. However, previous studies comparing blood gas analyzers of different brands have shown that the measures obtained from ABG machines are highly statistically concordant .
Another limitation was that the ABG samples were collected using conventional syringes containing liquid heparin. The use of dried heparin syringes  could improve the accuracy of the results by decreasing the dilution of the sample.
Previous studies referenced earlier have shown that ABG machines do not measure electrolytes accurately, but since all these studies compared results between AA and ABG analyzers in different environments, we created another limitation for ourselves by locating the ABG analyzer in the central laboratory in an environment similar to the AA, and in doing so we did undermine the true meaning of point-of-care testing, which is often equated to bed-side testing. However, the time gain was significant even by following the above practice since the time for analysis itself was minimized.
In conclusion, we advocate the use of ABG machines for adjudging serum potassium values accurately, in the absence of other specialized point-of-care analyzers. Further research is needed to establish their accuracy for sodium values.
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